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The aim of this study was to investigate antenatal glucocorticoids administration effect on gene expression of basic fibroblast growth factor, platelet-derived growth factor transforming growth factor-beta1 in congenital diaphragmatic hernia
Abstract:
BACKGROUND/PURPOSE: There is increasing evidence to suggest that various growth factors play a crucial role in fetal lung growth and morphogenesis. An array of peptide growth factors regulate cell proliferation, differentiation, and various other cell functions in the developing lung. The aim of this study was to investigate the effect of antenatal glucocorticoids administration on gene more...
Categories:
antenatal glucocorticoids administration; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 35 Page: 109-12 January 2000
T. Oue; H. Shima; Y. Taira; P. Puri
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The aim of this study was to test the hypothesize that administration of adriamycin to chick embryos would cause similar anomalies to the vertebral, anorectal, cardiac, tracheoesophageal, renal, limb anomalies association seen in the Adriamycin rat mode
Abstract:
Adriamycin is an anthracycline, anti-neoplastic drug with known teratogenic effects on foetal rats in what is known as the Adriamycin rat model (ARM). This includes conditions similar to those in newborn humans, known collectively as the VACTERL association. This comprises vertebral (V), anorectal (A), cardiac (C), tracheoesophageal (TE), renal (R) and limb (L) anomalies. We designed more...
Categories:
adriamycin; VACTERL malformations - vertebral, anorectal, cardiac, tracheoesophageal, renal, limb anomalies
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 19 Page: 359-64 July 2003
A. Mortell; J. Giles; J. Bannigan; P. Puri
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The aim of this study was to test the hypothesis that Shh-expressing ectopic notochord could contribute to an increased volume of notochord relative to total embryo volume, resulting in an increased concentration of Shh in the notochord of embryos
Abstract:
BACKGROUND/PURPOSE: The Adriamycin rat model (ARM) is a well-established model of the Vertebral, Anorectal, Cardiac, Tracheoesophageal, Renal, Limb (VACTERL) association. The notochord, which expresses Sonic Hedgehog (Shh), has been found to be grossly malformed with ventral ectopic branches in the foregut region of embryos in the ARM. The authors designed this study to test the more...
Categories:
Shh-expressing ectopic notochord; total embryo volume
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 39 Page: 859-63 June 2004
A. Mortell; A. M. O'Donnell; S. Giles; J. Bannigan; P. Puri
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The aim of this study was to test the hypothesize that the functional changes in the bladder following bladder outlet obstruction are due to alteration in cholinergic and sensory neuropeptide innervation
Abstract:
Posterior urethral valves (PUV) are the most common cause of bladder outlet obstruction (BOO) in infancy. Bladder instability, poor compliance and myogenic failure are responsible for the poor long-term prognosis in these patients. Previous studies have reported abundance of sensory neuropeptides, e.g. substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal more...
Categories:
functional changes in the bladder; bladder outlet obstruction; neuropeptide innervation
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 19 Page: 427-31 August 2003
B. Chertin; U. Rolle; S. Cascio; P. Puri
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The aim of this study was to determine the expression of Ag-II receptor subtypes and TGF-beta1 in the hypoplastic heart of nitrofen-induced congenital diaphragmatic hernia
Abstract:
The renin-angitensin system (RAS) plays an important role as a growth factor in cardiac development. Angiotensin converting enzyme is involved in converting angiotensin I to angiotensin II (Ag-II). The effects of Ag-II are mediated by two primary receptors, type 1 (AT1) and type 2 (AT2). Ag-II stimulates transforming growth factor-beta1(TGF-beta1) and acts as a potent stimulant of more...
Categories:
hypoplastic heart; congenital diaphragmatic hernia
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 21 Page: 148-52 March 2005
H. Teramoto; M. Shinkai; P. Puri
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The aim of this study was to investigate gene level expression of TR-alpha1 and TR-beta1 in the lung of nitrofen-induced congenital diaphragmatic hernia using reverse transcription polymerase chain reaction (RT-PCR)
Abstract:
PURPOSE: Thyroid receptor alpha-1 (TR-alpha1) and thyroid receptor beta-1 (TR-beta1) are thought to be essential for the fetal and postnatal development of the lung. The authors investigated gene level expression of TR-alpha1 and TR-beta1 in the lung of nitrofen-induced congenital diaphragmatic hernia (CDH) using reverse transcription polymerase chain reaction (RT-PCR). METHODS: CDH was more...
Categories:
TR-alpha1 and TR-beta1; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 36 Page: 1675-8 November 2001
H. Teramoto; N. Guarino; P. Puri
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The aim of this study was to test the hypothesize that abnormal expression of renin-angiotensin system components may be responsible for renal hypoplasia in congenital diaphragmatic hernia
Abstract:
The association between renal hypoplasia and pulmonary hypoplasia in congenital diaphragmatic hernia (CDH) has become recently appreciated. However, the underlying mechanisms responsible for this association are still unknown. Renin-angiotensin system (RAS) plays an important role in renal and somatic growth, angiogenesis and reproduction. We hypothesized that abnormal expression of RAS more...
Categories:
renin-angiotensin system; congenital diaphragmatic hernia
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 22 Page: 57-60 January 2006
B. Chertin; N. Nakazawa; S. Montedonico; H. Shima; P. Puri
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The aim of this study was to investigate the effect of antenatal glucocorticoid administration on DNA synthesis and RNA and protein content in nitrofen-induced congenital diaphragmatic hernia in rats
Abstract:
Antenatal glucocorticoids treatment has been shown to correct pulmonary immaturity. The thymidine analog bromodeoxyuridine (BrdU) is incorporated into S-phase cells and used as a marker of DNA synthesis. In this study, we investigated the effect of antenatal glucocorticoid administration on DNA synthesis and RNA and protein content in nitrofen-induced congenital diaphragmatic hernia more...
Categories:
antenatal glucocorticoid; DNA synthesis; congenital diaphragmatic hernia
Geography:
Republic of Ireland
Pediatr Res,
Vol: 48 Page: 789-93 December 2000
T. Oue; H. Shima; N. Guarino; P. Puri
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The aim of this study was to investigate the effect of antenatal glucocorticoid therapy on smooth-muscle-cell DNA synthesis in the pulmonary arteries in a nitrofen-induced congenital diaphragmatic hernia rat model following nitrofen admin
Abstract:
The aim of this study was to investigate the effect of antenatal glucocorticoid therapy on smooth-muscle-cell (SMC) DNA synthesis in the pulmonary arteries (PA) in a nitrofen-induced congenital diaphragmatic hernia (CDH) rat model following nitrofen administration on day 9.5 of gestation. Antenatal dexamethasone (DEX) was given intraperitoneally on days 18.5 and 19.5 of gestation. more...
Categories:
antenatal glucocorticoid therapy; congenital diaphragmatic hernia
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 16 Page: 414-6 2000
Y. Taira; H. Shima; E. Miyazaki; K. Ohshiro; P. Puri
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The aim of this study was to evaluate the effect of antenatal glucocorticoid administration on ET-1 system in nitrofen-induced congenital diaphragmatic hernia hypoplastic lung in rats
Abstract:
BACKGROUND/PURPOSE: The hypoplastic lung and persistent pulmonary hypertension (PPH) are the principle causes of high mortality and morbidity in infants with congenital diaphragmatic hernia (CDH). Endothelin-1 (ET-1), which is produced by vascular endothelial cells and some leukocytes, plays a key role in modulating pulmonary vascular tone in PPH. Two different receptors (ET(A) and more...
Categories:
antenatal glucocorticoid; congenital diaphragmatic hernia lung
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 35 Page: 203-7 February 2000
H. Shima; T. Oue; Y. Taira; E. Miyazaki; P. Puri
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The aim of this study was to determine cardiac gene expression of ET-1 and of its receptor ET(A) and the cardiac ET-1 content in the heart of nitrofen-induced congenital diaphragmatic hernia in rats and evaluate antenatal Dexamethason treatment effects
Abstract:
BACKGROUND/PURPOSE: Although high levels of endothelin-1 (ET-1) in plasma may be relevant in certain pathophysiologic states, such as pulmonary hypertension accompanying congenital diaphragmatic hernia (CDH), experimental evidence favors a local, paracrine, or autocrine role for ET-1 in most tissues. Evidence of ET-1 production has been documented in fetal heart tissue where it exerts more...
Categories:
ET-1 and receptor ET(A) receptor; congenital diaphragmatic hernia;
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 37 Page: 1563-7 November 2002
N. Guarino; P. Puri
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The aim of this study was to investigate whether maternal administration of dexamethasone has any effect on the expression of surfactant protein A and surfactant protein B in nitrofen-induced experimental congenital diaphragmatic hernia rat model
Abstract:
BACKGROUND/PURPOSE: Pulmonary hypoplasia is one of the main causes for the high mortality rate in patients with congenital diaphragmatic hernia (CDH). The expression of surfactant protein A in the hypoplastic CDH lung is reduced, and its concentration is decreased in the amniotic fluid of pregnancies complicated by CDH. In a CDH experimental model, prenatal glucocorticoid treatment has more...
Categories:
maternal administration of dexamethason; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 35 Page: 1468-73 October 2000
N. Guarino; T. Oue; H. Shima; P. Puri
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The aim of this study was to investigate the effect of antenatal glucocorticoid administration on the atrial natriuretic peptide system in nitrofen-induced congenital diaphragmatic hernia hypoplastic lung in rats.
Abstract:
Atrial natriuretic peptide (ANP) plays a major role in electrolyte and volume homeostasis through potent biological effects including vasorelaxation, bronchorelaxation, lung permeability, and clearance. There are two distinct biochemical and functional classes of ANP receptors, guanylate cyclase receptor (GC-R) and clearance receptors (clearance-R). Two subtypes of GC-R have been more...
Categories:
antenatal glucocorticoid; atrial natriuretic peptide system; congenital diaphragmatic hernia hypoplastic lung
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 16 Page: 252-5 2000
H. Shima; N. Guarino; P. Puri
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The aim of this study was to investigate whether molecular mimicry by Helicobacter mustelae causes autoantibodies in infected ferrets
Abstract:
Infection with Helicobacter pylori has been associated with induction of autoantibodies that cross-react with the gastric mucosa. There have been discordant reports as to whether or not these autoantibodies arise due to molecular mimicry between H. pylori and host cell antigens on parietal cells. In this study, we investigated whether molecular mimicry by H. mustelae causes more...
Categories:
Helicobacter mustelae; autoantibodies
Geography:
Republic of Ireland
Infect Immun,
Vol: 69 Page: 2708-13 April 2001
T. O. Croinin; M. Clyne; B. J. Appelmelk; B. Drumm
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The aim of this study was to test the hypothesis that Adriamycin administration during a particular time frame could yield a high incidence of urinary tract anomalies
Abstract:
Congenital obstructive uropathy is associated with significant morbidity and mortality in the human neonate. The pathophysiology of congenital obstructive uropathy is poorly understood. There are very few experimental models of prenatal obstruction of the urinary tract, except in the fetal lamb or inbred rats. Prenatal exposure to Adriamycin in a rat model leads to a spectrum of more...
Categories:
adriamycin administration; urinary tract anomalies
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 21 Page: 212-6 March 2005
A. Mortell; L. Fourcade; V. Solari; P. Puri
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The aim of this study was to determine the gene and protein levels of atrial natriuretic peptide in the heart of nitrofen-induced congenital diaphragmatic hernia in rats and to evaluate the effect of antenatal dexamethazone treatment
Abstract:
BACKGROUND/PURPOSE:The aim of this study was to determine the gene and protein levels of atrial natriuretic peptide (ANP) in the heart of nitrofen-induced congenital diaphragmatic hernia (CDH) in rats and to evaluate the effect of antenatal dexamethazone (Dex) treatment. METHODS: CDH model was induced in pregnant rats after administration of 100 mg of nitrofen on day 9.5 of gestation more...
Categories:
antenatal dexamethazone treatment; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 36 Page: 1497-501 October 2001
N. Guarino; H. Shima; P. Puri
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The aim of this study was to investigate the hypothesise of increased cell death in adriamycin-exposed embryos
Abstract:
The adriamycin rat model (ARM) exhibits many features of the VACTERL association. Adriamycin is a cytotoxic drug used in cancer chemotherapy. Although its exact mode of action is not clear, it is presumed to have a similar cytotoxic role in the developing embryo. Lysotracker red (LT) is a dye that stains phagolysosomes and apoptotic bodies and allows entire rodent embryos to be stained more...
Categories:
adriamycin-exposed embryos; increased cell death;
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 18 Page: 576-80 October 2002
J. Gillick; S. Giles; J. Bannigan; P. Puri
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The aim of this study was to test the hypothesize that the hyperreactivity of the newborn pulmonary vasculature in congenital diaphragmatic hernia might reflect a relatively low level of smooth muscle K(+) channel activity because of hypoxemia
Abstract:
The newborn with congenital diaphragmatic hernia (CDH) is at high risk of developing persistent pulmonary hypertension (PPH). Recently, smooth muscle K(+) channels have been implicated in hypoxic pulmonary vasoconstriction in adults. We hypothesized that the hyperreactivity of the newborn pulmonary vasculature in CDH might reflect a relatively low level of smooth muscle K(+) channel more...
Categories:
smooth muscle K(+) channel activity; hyperreactivity; congenital diaphragmatic hernia
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 20 Page: 192-6 March 2004
M. Sakai; K. Unemoto; V. Solari; P. Puri
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The aim of this study was to describe the morphology and functional importance of the autonomic nervous system in the upper urinary tract
Abstract:
AIMS: The morphology and functional importance of the autonomic nervous system in the upper urinary tract is still not completely understood. Previous histological studies investigating the innervation of the urinary tract have mainly used conventional sections in which the three-dimensional structure of the intramural innervation is difficult to achieve. In contrast, the whole-mount more...
Categories:
the autonomic nervous system; upper urinary tract
Geography:
Republic of Ireland
Neurourol Urodyn,
Vol: 27 Page: 341-7 2008
U. Rolle; E. Brylla; B. Tillig; B. Chertin; S. Cascio; P. Puri
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The aim of this study was to investigate the distribution of nitrergic and cholinergic innervation in rabbit, pig, and human upper urinary tract
Abstract:
To investigate the distribution of nitrergic and cholinergic innervation in rabbit, pig, and human upper urinary tract, (UUT) whole-mount preparations and frozen sections were stained with nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase and acetylcholinesterase (AChE) histochemistry. NADPH-diaphorase and AChE staining demonstrated two neuronal plexuses in the submucous more...
Categories:
nitrergic and cholinergic innervation; upper urinary tract
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 18 Page: 315-8 September 2002
U. Rolle; B. Chertin; L. Nemeth; P. Puri
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The aim of this study was to determine the normal nitrergic neuronal density and morphology in the submucosal plexus of the porcine distal bowel from fetal life to adulthood
Abstract:
BACKGROUND/PURPOSE: As our understanding of the enteric nervous system improves, it becomes clear that it is no longer sufficient to simply determine whether enteric ganglion cells are present but also to determine whether correct number and types of ganglion cells are present. Nitric oxide is recognized as a potent mediator of inhibitory nerves responsible for the relaxation of the more...
Categories:
nitrergic neuronal morphology; submucosal plexus of the bowel;
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 41 Page: 1029-35 May 2006
S. Montedonico; T. Sri Paran; M. Pirker; U. Rolle; P. Puri
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The aim of this study was to investigate the age-related changes in the sympathetic innervation of the myenteric plexus
Abstract:
Knowledge about the foetal development of the normal enteric nervous system (ENS) is crucial for the understanding of congenital and acquired functional abnormalities of the gut. The ENS is the largest and most complex division of the peripheral nervous system and consists of intrinsic and extrinsic components. Although previous studies have described sympathetic innervation of the more...
Categories:
sympathetic innervation of the myenteric plexus;
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 23 Page: 659-63 July 2007
T. Sri Paran; U. Rolle; P. Puri
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The aim of this study was to investigate differences in nitrergic innervation of the developing chick cloaca and colorectum
Abstract:
The intrinsic innervation of the developing gut has long been a subject of investigation, but little is known regarding that of the embryonic cloaca. The cloaca, like the rest of the gastrointestinal tract, is intrinsically innervated by the enteric nervous system. Nitrergic neurons and fibres make up a large part of this system, thus, their distribution provides us with a useful more...
Categories:
nitrergic innervation
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 22 Page: 90-4 January 2006
A. M. O'Donnell; J. Bannigan; P. Puri
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The aim of this study was to test the hypothesize that expression of GATA4 and GATA6 is reduced in early stages of gestation in a congenital diaphragmatic hernia hypoplastic heart
Abstract:
PURPOSE: The high incidence of cardiac malformations in humans and animal models with congenital diaphragmatic hernia (CDH) is well known. The precise molecular mechanisms underlying cardiac maldevelopment in CDH are still unclear. It has been reported that GATA4 and GATA6, members of the GATA transcription factor family, act cooperatively to regulate cardiovascular development, and the more...
Categories:
GATA4 and GATA6; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 43 Page: 362-6 February 2008
H. Takayasu; H. Sato; K. Sugimoto; P. Puri
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The aim of this study was to test the hypothesize that Wnt-mediated signaling is altered in nitrofen-induced hypoplastic lungs
Abstract:
PURPOSE: The pathogenesis of pulmonary hypoplasia associated with congenital diaphragmatic hernia is poorly understood. Recently, it has been reported that Wnt signaling pathway plays a critical role in branching lung morphogenesis. Mice lacking Wnt7b gene die soon after birth because of respiratory failure and display severe lung hypoplasia. Wnt2 gene is expressed in the distal airway more...
Categories:
Wnt-mediated signaling; hypoplastic lungs
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 42 Page: 426-30 February 2007
H. Takayasu; N. Nakazawa; S. Montedonico; P. Puri
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The aim of this study was to investigate SP mRNA expression in hypoplastic congenital diaphragmatic hernia lung in rats during mechanical ventilation in order to determine the effect of O2 on SP synthesis in congenital diaphragmatic hernia
Abstract:
The hypoplastic lung in congenital diaphragmatic hernia (CDH) has both a quantitative and qualitative reduction in surfactant. Recently, the role of oxygen (O2) as a regulator of pulmonary surfactant-associated protein (SP) gene expression has been reported. The mRNA level of SP has been demonstrated to be increased in the lungs of animals exposed to hyperoxia. The aim of this study was more...
Categories:
SP mRNA expression; congenital diaphragmatic hernia lung
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 16 Page: 473-7 2000
H. Shima; N. Guarino; P. Puri
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The aim of this study was to investigate the expression and production of elastin in the lung in newborn rats with congenital diaphragmatic hernia during mechanical ventilation
Abstract:
BACKGROUND/PURPOSE: Hypolastic lung in congenital diaphragmatic hernia (CDH) shows markedly thickened alveolar walls, increased interstitial tissue, and markedly diminished alveolar air space, showing morphologic immaturity. Decrease in lung compliance and distensibility often is seen in human CDH as well as experimentally produced CDH. Collagen and elastin, critical components of the more...
Categories:
mechanical ventilation; elastin; congenital diaphragmatic hernia;
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 37 Page: 1253-7 September 2002
N. Guarino; H. Teramoto; H. Shima; T. Oue; P. Puri
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The aim of this study was to investigate the effect of nitric oxide on fibroblast growth factor-10 and bone morphogenetic protein 4 expressions in the branching morphogenesis of fetal rat lung explants
Abstract:
PURPOSE: Nitric oxide (NO) can accelerate branching morphogenesis of fetal rat lung explants in vitro, whereas its exact mechanism remains unclear. In this study, we investigate the effect of NO on the expression of fibroblast growth factor-10 (FGF10) and bone morphogenetic protein-4 (BMP4), which plays an important role in bud formation. METHODS: Fetal rat lungs harvested on day 13.5 more...
Categories:
nitric oxide; fibroblast growth factor-10; branching morphogenesis of the lung
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 40 Page: 1030-3 June 2005
M. Shinkai; T. Shinkai; M. A. Pirker; S. Montedonico; P. Puri
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The aim of this study was to evaluate the effect of nitric oxide on the morphogenesis of hypoplastic lung using nitrofen-induced rat lung explant model
Abstract:
BACKGROUND/PURPOSE: Nitric oxide (NO) is an important cell-signaling molecule, and its generators, nitric oxide synthases, are expressed temporospatially in fetal rat lung. Recently, NO has been reported to modulate branching of the fetal rat lung lobe in vitro. We designed this study to evaluate the effect of NO on the morphogenesis of hypoplastic lung using nitrofen-induced rat lung more...
Categories:
nitric oxide; hypoplastic lung
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 40 Page: 17-21 January 2005
M. Shinkai; T. Shinkai; M. E. Pirker; S. Montedonico; P. Puri
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The aim of this study was to test the hypothesize that vascular endothelial growth factor may accelerate branching morphogenesis and thus may modulate lung growth in normal and nitrofen-induced pulmonary hypoplastic lungs
Abstract:
PURPOSE: Changes in vascular structures as well as vascular endothelial growth factor (VEGF) downregulation have been reported in hypoplastic lungs associated with congenital diaphragmatic hernia. We hypothesized that VEGF may accelerate branching morphogenesis and thus may modulate lung growth in normal and nitrofen-induced pulmonary hypoplastic lungs. METHODS: A hypoplastic fetal lung more...
Categories:
endothelial growth factor; pulmonary hypoplastic lungs
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 41 Page: 781-6 April 2006
M. Shinkai; T. Shinkai; S. Montedonico; P. Puri
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The aim of this study was to investigate the clinical disorder of recessive congenital methemoglobinemia
Abstract:
The clinical disorder of recessive congenital methemoglobinemia (RCM, OMIN 250800) is associated with mutations in NADH:cytochrome b5 reductase (cb5r) and manifests as cyanosis from birth. Screening a cyanotic infant indicated elevated methemoglobin levels and decreased cb5r activity suggesting RCM. Sequencing the DIA1 gene encoding cb5r revealed a novel mutation, C27161T (NCBI more...
Categories:
congenital methemoglobinemia
Geography:
Northern Ireland
Arch Biochem Biophys,
Vol: 447 Page: 59-67 March 2006
M. J. Percy; L. J. Crowley; J. Boudreaux; M. J. Barber
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The aim of this study was to investigate mRNA expression of ET-1, eNOS, and CGRP in congenital diaphragmatic hernia lung in the perinatal period
Abstract:
The high mortality in patients with congenital diaphragmatic hernia (CDH) has been attributed to pulmonary hypoplasia and persistent pulmonary hypertension (PPH). Endothelin-1 (ET-1), nitric oxide (NO), and calcitonin gene-related peptide (CGRP) have been reported to be important vasoactive mediators in the perinatal pulmonary circulation. The exact mechanism by which these vasoactive more...
Categories:
mRNA expression; congenital diaphragmatic hernia lung
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 21 Page: 143-7 March 2005
T. Shinkai; H. Shima; V. Solari; P. Puri
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The aim of this study was to determine the gene level expression of fibroblast growth factors-10 and FGF-7 in the lung of nitrofen-induced congenital diaphragmatic hernia
Abstract:
BACKGROUND/PURPOSE: Newborns with congenital diaphragmatic hernia (CDH) still have a high mortality rate, which has been attributed to pulmonary hypoplasia and pulmonary hypertension. Fibroblast growth factors (FGFs) are essential components of the gene network that regulates lung development. Recent studies suggest that the new member of FGF family, FGF-10, plays a fundamental role in more...
Categories:
gene level expression; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 38 Page: 1021-4 July 2003
H. Teramoto; A. Yoneda; P. Puri
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The aim of this study was to determine the gene-level expression of insulin-like growth factor-1 and epidermal growth factor in the hearts of rats with nitrofen-induced congenital diaphragmatic hernia
Abstract:
Newborns with congenital diaphragmatic hernia (CDH) still have high mortality. Recently, a possible role of cardiac maldevelopment has been suggested. Human and experimental studies have demonstrated that heart weight is significantly reduced in the presence of CDH. Recent studies have suggested an important role for insulin-like growth factor-I (IGF-I) in the regulation of cardiac more...
Categories:
insulin-like growth factor-1; epidermal growth factor; congenital diaphragmatic hernia
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 17 Page: 284-7 May 2001
H. Teramoto; P. Puri
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The aim of this study was to determine if Glutathione S-transferase levels correlated with histologic changes of intestinal ischemia in a controlled animal model of mesenteric intestinal ischemia
Abstract:
BACKGROUND/PURPOSE: The accurate and early diagnosis of intestinal ischemia remains difficult chiefly because of a lack of a suitable marker that is noninvasive and easy to use. The glutathione S-transferases (GST) are a family of cytosolic enzymes involved in detoxification and released from a variety of cells when the cell membrane is damaged. The enzymes are distributed widely in the more...
Categories:
Glutathione S-transferase levels; mesenteric intestinal ischemia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 37 Page: 1543-8 November 2002
S. Khurana; M. T. Corbally; F. Manning; T. Armenise; B. Kierce; C. Kilty
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The aim of this study was to evaluate the effects of supplementing pregnant ewe's diet with high levels of minerals and vitamin E on immunoglobulin G absorption by the lamb and if any altered efficacy of IgG absorption was due to colostrum or the lamb
Abstract:
A 2 x 2 factorial arrangement of treatments with 78 mature ewes was used to evaluate the effects of supplementing the pregnant ewe's diet with high levels of minerals and vitamin E on immunoglobulin G (IgG) absorption by the lamb and whether any altered efficacy of IgG absorption was due to the colostrum or to the lamb. The ewes were estrus-synchronized in October and housed in wk 10 of more...
Categories:
dietary supplementation immunoglobulin G absorption
Geography:
Republic of Ireland
J Anim Sci,
Vol: 83 Page: 871-8 April 2005
T. M. Boland; N. Keane; P. Nowakowski; P. O. Brophy; T. F. Crosby
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The aim of this study was to investigate molecular markers for AECs-I [ICAM-1, T1alpha, aquaporin 5 ] and molecular markers for AECs-II [thyroid transcription factor-1), surfactant protein in the nitrofen-induced congenital diaphragmatic hernia lung
Abstract:
Pulmonary hypoplasia is the principal cause of morbidity and mortality in infants with congenital diaphragmatic hernia (CDH). Still, relatively little is known about the mechanisms causing lung hypoplasia associated with CDH. The differentiation from alveolar epithelial cells type II (AECs-II) into alveolar epithelial cells type I (AECs-I) is one of the key processes in lung development more...
Categories:
molecular markers; congenital diaphragmatic hernia lung
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 23 Page: 405-10 May 2007
H. Takayasu; N. Nakazawa; S. Montedonico; K. Sugimoto; H. Sato; P. Puri
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The aim of this study was to investigate pulmonary ICAM-1 and VCAM-1 expression in congenital diaphragmatic hernia lung in rats and to determine the effect of antenatal glucocorticoid
Abstract:
Recently, increased expression of inflammatory cytokine, tumor necrosis factor (TNF)-alpha, has been reported in both humans and animal models with CDH and the decreased TNF-alpha expression in CDH lung after antenatal dexamethasone (Dex) treatment. Intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 are induced by several inflammatory cytokines such as more...
Categories:
antenatal glucocorticoid; congenital diaphragmatic hernia lung
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 19 Page: 365-70 July 2003
K. Unemoto; M. Sakai; H. Shima; N. Guarino; P. Puri
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The aim of this study was to examine the hypothesis that adriamycin administration could promote upregulation of FN expression increasing gut-notochord adherence and development of abnormal ventral notochordal branching in the Adriamycin rat model
Abstract:
BACKGROUND: The VACTERL association is a spectrum of clinical conditions, including esophageal atresia (EA) and tracheoesophageal fistula (TEF), which affects approximately 1 in 5,000 live human births. The administration of intraperitoneal Adriamycin to pregnant rats reliably induces anomalies, such as EA and TEF, in their offspring, in what is known as the Adriamycin rat model (ARM). more...
Categories:
adriamycin; abnormal ventral notochordal branching
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 20 Page: 276-82 April 2004
A. Mortell; A. M. O'Donnell; S. Giles; J. Bannigan; P. Puri
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The aim of this study was to investigate mRNA and protein levels of vascular endothelial growth factor in congenital diaphragmatic hernia lung and to determine whether antenatal Dex treatment has any effect Vascular endothelial growth factor production
Abstract:
Persistent pulmonary hypertension (PPH) in congenital diaphragmatic hernia (CDH) lung has been shown to be associated with structural changes in the pulmonary vasculature, including medial and adventitial thickening. Vascular endothelial growth factor (VEGF) is a potent mitogenic and permeability factor targeting predominantly endothelial cells. mRNA encoding VEGF is detected in all more...
Categories:
mRNA and protein levels; congenital diaphragmatic hernia lung; vascular endothelial growth factor
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 18 Page: 221-6 May 2002
T. Oue; A. Yoneda; H. Shima; Y. Taira; P. Puri
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The aim of this study was to describe Bordetella pertussis respiratory infection, its expression of mRNA transcripts for IL-1beta and TNFalpha and transient expression of IL-6 in the hippocampus and hypothalamus
Abstract:
Parenteral injection of endotoxin has been used as a model to examine the role of pro-inflammatory cytokines in the centrally controlled responses to Gram-negative bacterial infection. However, the events that occur following mucosal exposure to live bacteria have received little attention. In this study, we have used a murine model to demonstrate that respiratory infection with more...
Categories:
Bordetella pertussis respiratory infection; hippocampus and hypothalamus
Geography:
Republic of Ireland
J Neuroimmunol,
Vol: 102 Page: 172-81 January 2000
C. E. Loscher; S. Donnelly; M. A. Lynch; K. H. Mills
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The aim of this study was to compare the mechanisms of Cryptosporidium parvum and Cryptosporidium hominis invasion using a primary cell model of infection
Abstract:
Cryptosporidiosis in humans is caused by the zoonotic pathogen Cryptosporidium parvum and the anthroponotic pathogen Cryptosporidium hominis. To what extent the recently recognized C. hominis species differs from C. parvum is unknown. In this study we compared the mechanisms of C. parvum and C. hominis invasion using a primary cell model of infection. Cultured primary bovine and human more...
Categories:
Cryptosporidium parvum; Cryptosporidium hominis; mechanisms of infection
Geography:
Republic of Ireland
Infect Immun,
Vol: 74 Page: 99-107 January 2006
A. Hashim; G. Mulcahy; B. Bourke; M. Clyne
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The aim of this study was to investigate if key apoptosis regulating proteins are down-regulated during postnatal tissue development
Abstract:
The intrinsic apoptotic pathway is essential for murine development. We have previously shown that key mediators of this pathway, such as Bim, Apaf-1 and caspase-3, are down-regulated during the postnatal development of the retina. In this study, we demonstrate that this expression pattern is a feature of other distinct tissues such as the brain, heart and skeletal muscle. Caspase-9 more...
Categories:
apoptosis regulating proteins; postnatal tissue development
Geography:
Republic of Ireland
Int J Dev Biol,
Vol: 51 Page: 415-23 2007
S. D. Madden; M. Donovan; T. G. Cotter
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The aims of this study were to investigate the effects of pulmonary hypoplasia on renal development and to evaluate retinoids status of kidneys in the nitrofen model of congenital diaphragmatic hernia
Abstract:
BACKGROUND/PURPOSE: The relationship of the developing lung and kidney is not completely understood. Renal enlargement has been reported in association with pulmonary hypoplasia in congenital diaphragmatic hernia (CDH). Recent studies suggest that retinoids may be involved in the pathogenesis of CDH. The aims of this study were to investigate the effects of pulmonary hypoplasia on renal more...
Categories:
pulmonary hypoplasia; renal development; congenital diaphragmatic hernia
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 42 Page: 239-43 January 2007
S. Montedonico; N. Nakazawa; T. Shinkai; J. Bannigan; P. Puri
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The aim of this study was to undertake a genome scan using 187 microsatellite genetic markers and all backcross animals were phenotyped at birth for the presence and severity of arthrogryposis
Abstract:
Arthrogryposis is a congenital malformation affecting the limbs of newborn animals and infants. Previous work has demonstrated that inherited ovine arthrogryposis (IOA) has an autosomal recessive mode of inheritance. Two affected homozygous recessive (art/art) Suffolk rams were used as founders for a backcross pedigree of half-sib families segregating the IOA trait. A genome scan was more...
Categories:
genome scan; genetic markers; arthrogryposis
Geography:
Republic of Ireland
Mamm Genome,
Vol: 18 Page: 43-52 January 2007
A. M. Murphy; D. E. MacHugh; S. D. Park; E. Scraggs; C. S. Haley; D. J. Lynn; M. P. Boland; M. L. Doherty
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The aim of this study was to determine the incidence and histopathologic findings of multiple gastrointestinal atresias in order to understand the pathogenesis
Abstract:
Multiple gastrointestinal atresias (MGA) have been reported to account for 6% to 32% of all intestinal atresias. Controversy exists regarding the pathogenesis. Many investigators believe MGA to be the result of multiple ischemic infarctions of the intestinal tract. However, some have suggested that MGA results from a malformative process early in fetal life. Prenatal exposure to more...
Categories:
gastrointestinal atresias
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 17 Page: 361-4 July 2001
L. Fourcade; H. Shima; E. Miyazaki; P. Puri
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The aim of this study was to examine the age-related changes in the nitrergic innervation of the ileocecal valve to gain insights into the pathogenesis of intussusception
Abstract:
PURPOSE: Ileocecal intussusception is a relatively common surgical emergency in infants and young children. The etiology of intussusception is not clearly understood. Nitric oxide (NO) is a major inhibitory neurotransmitter in the enteric nervous system, which causes relaxation of the smooth muscles. In a lipopolysaccharide-induced experimental model of intussusception, altered more...
Categories:
nitrergic innervation of the ileocecal valve; intussusception
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 42 Page: 1515-9 September 2007
T. Cserni; S. Paran; P. Puri
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The aim of this study was to assess notochord volume sequentially from gestational days 10 to 14 to test the hypothesis that notochord hypertrophy occurs maximally soon after Adriamycin administration
Abstract:
BACKGROUND/PURPOSE: The Adriamycin rat model (ARM) is a well-established model of the vertebral, anorectal, cardiac, tracheoesophageal, renal, and limb association. An important finding in the ARM is that Adriamycin induces abnormal notochord morphology in the region of the foregut. Having recently demonstrated notochord hypertrophy in ARM embryos, the authors designed this study to more...
Categories:
notochord volume; Adriamycin
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 40 Page: 403-6 February 2005
A. Mortell; J. Gillick; S. Giles; J. Bannigan; P. Puri
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The aim of this study was to test the hypothesize that notochord anomalies allow ectopic expression of molecular signals in the developing embryo and thus lead to VACTERL malformations
Abstract:
BACKGROUND/PURPOSE: The Adriamycin rat model (ARM) is a reliable model of the VACTERL association. The notochord is structurally abnormal in the region of the foregut, midgut, and hindgut in the ARM. The authors hypothesised that notochord anomalies allow ectopic expression of molecular signals in the developing embryo and thus lead to VACTERL malformations. This study was designed to more...
Categories:
notochord anomalies; VACTERL malformations - Vertebral Anal Cardiac Fistula +/- eosophageal atresia : Renal or Radius Limb anomalies
Geography:
Republic of Ireland
J Pediatr Surg,
Vol: 38 Page: 469-73; discussion 469-73 March 2003
J. Gillick; E. Mooney; S. Giles; J. Bannigan; P. Puri
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The aim of this study was to investigate the development of enteric mucosal innervation in the pig at various ages of life
Abstract:
Knowledge regarding the foetal and postnatal development of the enteric nervous system is crucial for the understanding of congenital disorders. While lot of information exists regarding the myenteric and submucosal plexuses, the development of the mucosal plexus has not been previously studied. The mucosal innervation seems to play an important role in the local reflex activity of the more...
Categories:
enteric mucosal innervation
Geography:
Republic of Ireland
Pediatr Surg Int,
Vol: 22 Page: 997-1001 December 2006
T. S. Paran; U. Rolle; P. Puri
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The aim of this study was to analyzed the development of smooth muscle components of the ureterovesical junction and their nerve supply in the fetal, newborn and adolescent pig
Abstract:
PURPOSE: The mechanisms underlying functional maturation of the ureterovesical junction during infancy are still not fully understood. We analyzed the development of smooth muscle components of the ureterovesical junction and their nerve supply in the fetal, newborn and adolescent pig. MATERIALS AND METHODS: Bladder specimens were obtained from porcine fetuses at gestational ages 60 more...
Categories:
ureterovesical junction; nerve supply in the fetal
Geography:
Republic of Ireland
J Urol,
Vol: 177 Page: 1546-51 April 2007
M. E. Pirker; U. Rolle; T. Shinkai; M. Shinkai; P. Puri
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