Irish Child Health Database - Peer Reviewed Papers
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Cyclooxygenase-2 up-regulation in reflux nephropathy
Published in:
J Urol, Vol: 170, Page: 1624-7
Publication Date:
October 2003
Aims & Objectives:
The aim of this study was to examine COX-2 expression in the kidneys of children with reflux nephropathy
Abstract:
PURPOSE: Reflux nephropathy (RN) is a major cause of end stage renal failure in children and hypertension is a frequent complication. Cyclooxygenase-2 (COX-2) is an enzyme responsible for the prostaglandin synthesis. It has been shown that COX-2 up-regulates renin production leading to renovascular hypertension. We investigate COX-2 expression in the kidneys of children with RN. MATERIALS AND METHODS: Kidney specimens from 12 patients 2 to 13 years old with severe RN secondary to primary high grade vesicoureteral reflux obtained at the time of nephrectomy and 5 controls were examined. Single labeled immunohistochemistry was performed with 2 COX-2 antibodies using light and confocal microscopy. Quantification of COX-2 was determined by Western blotting analysis. COX-2 gene expression was evaluated by real-time quantitative reverse transcription polymerase chain reaction. RESULTS: There was a strong COX-2 immunoreactivity in the proximal tubules and tubulointerstitial space in the RN samples compared to controls. Immunoreactive COX-2 protein expression was markedly increased in RN samples compared to controls. Real-time reverse transcription polymerase chain reaction showed a significant increase in COX-2 mRNA expression in the RN samples compared to controls (p <0.05). CONCLUSIONS: Over expression of COX-2 in reflux nephropathy suggests that COX-2 may be involved in the pathogenesis of tubulointerstitial damage associated with severe reflux nephropathy.
Authors:
Study Type:
Study Papers » Case Control Study » Analytical Studies
Categories:
COX-2 expression; - reflux nephropathy
International Classification:
Diseases of the genitourinary system - reflux nephropathy
Keywords:
- Blotting, Western
- Child
- Child, Preschool
- Cyclooxygenase 2
- Female
- Gene Expression Regulation, Enzymologic
- Humans
- Hypertension, Renal/ genetics/pathology
- Infant
- Isoenzymes/ genetics
- Kidney Failure, Chronic/ genetics/pathology
- Kidney Tubules, Proximal/enzymology/pathology
- Male
- Membrane Proteins
- Nephritis, Interstitial/ genetics/pathology
- Prostaglandin-Endoperoxide Synthases/ genetics
- Reference Values
- Reverse Transcriptase Polymerase Chain Reaction
- Up-Regulation/genetics
- Vesico-Ureteral Reflux/ genetics/pathology
Geography:
Republic of Ireland (Ireland)