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Irish Child Health Database - Peer Reviewed Papers

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Nucleic Acid Denaturation

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1. Denaturing high-performance liquid chromatography using the WAVE DNA fragment analysis system

This article discusses denaturing high-performance liquid chromatography using the WAVE DNA fragment analysis system

Abstract:

Denaturing high-performance liquid chromatography (DHPLC) is a chromatographic mutation analysis technique that is based on temperature-dependent separation of DNA containing mismatched base pairs from polymerase chain reaction (PCR)-amplified DNA fragments. The WAVE system, developed for DHPLC analysis, allows for unattended analysis of 96 samples directly from a PCR plate under a more...

Geography:

Republic of Ireland

Methods Mol Med, Vol: 108 Page: 173-87 2005
E. Donohoe

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