Skip Navigation, or press ALT and K together and then press enter.Welcome to the OMC website. This site has been developed for both the visually impaired and non visually impaired. If you would like to use the visually impaired version of this site please go to, or press ALT and I together and then press enter

Irish Child Health Database - Peer Reviewed Papers

Database Search

You are here: Irish Child Health Database » Study Papers » Papers reporting secondary research » Animal Study
  • Demonstration of intrinsic innervation of the guinea pig upper urinary tract using whole-mount preparation

    Published in:

    Neurourol Urodyn, Vol: 27, Page: 341-7

    Publication Date:


    Aims & Objectives:

    The aim of this study was to describe the morphology and functional importance of the autonomic nervous system in the upper urinary tract


    AIMS: The morphology and functional importance of the autonomic nervous system in the upper urinary tract is still not completely understood. Previous histological studies investigating the innervation of the urinary tract have mainly used conventional sections in which the three-dimensional structure of the intramural innervation is difficult to achieve. In contrast, the whole-mount preparation technique is a suitable method for visualizing the distribution of the mesh-like neuronal networks within the urinary tract. METHODS: The distribution and regional variation of neurofilament (NF), tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), and substance P-immunoreactive (SP-IR) neurons, as well as acetylcholinesterase (AChE) and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d)-positive neurons were investigated using whole-mount preparations of the guinea pig upper urinary tract. RESULTS: Two distinct nervous plexuses were detected within the muscle layers containing NF, TH, ChAT, and SP-IR nerves. AChE-positive nerves were seen in all layers. Only moderate NADPH-d-positive innervation was found. Renal pelvis, upper and lower part of the ureter showed an overall increased innervation compared to the middle portion of the ureter. Ganglia were found at the pelviureteric border displaying NF and TH immunoreactivity. CONCLUSION: The whole-mount preparation technique provides an elegant method for assessing the three-dimensional architecture of ureteral innervation. The guinea pig upper urinary tract is richly supplied with adrenergic, cholinergic, nitrergic, and sensory nerves which suggest that the autonomous nervous system plays an important role in controlling ureteral motility and blood flow.


    U. Rolle; E. Brylla; B. Tillig; B. Chertin; S. Cascio; P. Puri

    Study Type:

    Study Papers » Animal Study » Papers reporting secondary research


    the autonomic nervous system; upper urinary tract



    Republic of Ireland (Republic of Ireland)